FAN (Free Amino Nitrogen by OPA) Kit Beer/Wort
PRODUCT DESCRIPTION
Inorganic Nitrogen – Ammonium through enzyme reacts with NADH α-ketoglutarate giving NAD+ and glutamate. The absorbance decrease of NADH solution, measured at 366 nm, is proportional to the quantity of amine Nitrogen present in the sample.
Organic Nitrogen – The α-amino Nitrogen reacts in presence of N-acetyl-L-cysteine with o-phthalaldehyde forming an isoindole derived. The difference of absorbance, measured at 366 nm (end point), is correlated with the quantity of Organic Nitrogen expressed in mg/L of Nitrogen.
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